LOCUS       40924_4050        7653 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pADGal4 2.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-1997) Technical Services, Stratagene, 11011 North 
            Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-1999) Technical Services, Stratagene, 11011 North 
            Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 7653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (06-NOV-1997) Technical Services, Stratagene, 11011 North Torrey 
            Pines Road, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JAN-1999) Technical Services, Stratagene, 11011 North Torrey 
            Pines Road, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Jan 25, 1999 this sequence version replaced gi:2695664.
FEATURES             Location/Qualifiers
     source          1..7653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             488..829
                     /codon_start=1
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     promoter        complement(905..923)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1310..1497
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1618..2709)
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2710..3114)
                     /label=LEU2 promoter
     primer_bind     complement(3156..3172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3180..3196)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3204..3234)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3249..3270)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3351..3806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3833..3937
                     /label=AmpR promoter
     enhancer        3939..4130
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      complement(4483..5071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5245..6102)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6103..6207)
                     /label=AmpR promoter
     rep_origin      6489..7653
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattagg
      841 atcctctgct agcagagaat tcaattctct aatgcttctc gagagtatta gtcgactcta
      901 gagccctata gtgagtcgta ttactgcaga gatctatgaa tcgtagatac tgaaaaaccc
      961 cgcaagttca cttcaactgt gcatcgtgca ccatctcaat ttctttcatt tatacatcgt
     1021 tttgccttct tttatgtaac tatactcctc taagtttcaa tcttggccat gtaacctctg
     1081 atctatagaa ttttttaaat gactagaatt aatgcccatc ttttttttgg acctaaattc
     1141 ttcatgaaaa tatattacga gggcttattc agaagctttg gacttcttcg ccagaggttt
     1201 ggtcaagtct ccaatcaagg ttgtcggctt gtctaccttg ccagaaattt acgaaaagat
     1261 ggaaaagggt caaatcgttg gtagatacgt tgttgacact tctaaataag cgaatttctt
     1321 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     1381 aagtgactct taggttttaa aacgaaaatt cttgttcttg agtaactctt tcctgtaggt
     1441 caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct ctaccggcat
     1501 gcccgaaatt cccctaccct atgaacatat tccattttgt aatttcgtgt cgtttctatt
     1561 atgaatttca tttataaagt ttatgtacaa atatcataaa aaaagagaat ctttttaagc
     1621 aaggattttc ttaacttctt cggcgacagc atcaccgact tcggtggtac tgttggaacc
     1681 acctaaatca ccagttctga tacctgcatc caaaaccttt ttaactgcat cttcaatggc
     1741 cttaccttct tcaggcaagt tcaatgacaa tttcaacatc attgcagcag acaagatagt
     1801 ggcgataggg tcaaccttat tctttggcaa atctggagca gaaccgtggc atggttcgta
     1861 caaaccaaat gcggtgttct tgtctggcaa agaggccaag gacgcagatg gcaacaaacc
     1921 caaggaacct gggataacgg aggcttcatc ggagatgata tcaccaaaca tgttgctggt
     1981 gattataata ccatttaggt gggttgggtt cttaactagg atcatggcgg cagaatcaat
     2041 caattgatgt tgaaccttca atgtaggaaa ttcgttcttg atggtttcct ccacagtttt
     2101 tctccataat cttgaagagg ccaaaacatt agctttatcc aaggaccaaa taggcaatgg
     2161 tggctcatgt tgtagggcca tgaaagcggc cattcttgtg attctttgca cttctggaac
     2221 ggtgtattgt tcactatccc aagcgacacc atcaccatcg tcttcctttc tcttaccaaa
     2281 gtaaatacct cccactaatt ctctgacaac aacgaagtca gtacctttag caaattgtgg
     2341 cttgattgga gataagtcta aaagagagtc ggatgcaaag ttacatggtc ttaagttggc
     2401 gtacaattga agttctttac ggatttttag taaaccttgt tcaggtctaa cactacctgt
     2461 accccattta ggaccaccca cagcacctaa caaaacggca tcaaccttct tggaggcttc
     2521 cagcgcctca tctggaagtg ggacacctgt agcatcgata gcagcaccac caattaaatg
     2581 attttcgaaa tcgaacttga cattggaacg aacatcagaa atagctttaa gaaccttaat
     2641 ggcttcggct gtgatttctt gaccaacgtg gtcacctggc aaaacgacga tcttcttagg
     2701 ggcagacatt agaatggtat atccttgaaa tatatatata tattgctgaa atgtaaaagg
     2761 taagaaaagt tagaaagtaa gacgattgct aaccacctat tggaaaaaac aataggtcct
     2821 taaataatat tgtcaacttc aagtattgtg atgcaagcat ttagtcatga acgcttctct
     2881 attctatatg aaaagccggt tccggcctct cacctttcct ttttctccca atttttcagt
     2941 tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa
     3001 tttgattctg tgcgatagcg cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg
     3061 ttgctaagag attcgaactc ttgcatctta cgatacctga gtattcccac agttggggat
     3121 ctcgactcta gctagaggat caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3241 ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc cgctttccag
     3301 tcgggaaacc tgtcgtgcca gttatatgcg gccgcataat ttaggcgcgt aaattgtaag
     3361 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3421 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3481 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3541 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3601 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3661 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3721 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3781 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     3841 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3901 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     3961 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4021 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4141 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4201 atttttttta tttatgcaga ggccgaggcc gcctcggcca ttacagctgg attaatgaat
     4261 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4321 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4381 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4441 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4921 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5221 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5281 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5341 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5401 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5461 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5521 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5581 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5641 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5701 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5761 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5821 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5881 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5941 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6001 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6061 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     6241 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     6301 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6361 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     6421 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     6481 gcaccataac gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata
     6541 tacaggcaac acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc
     6601 gttgcatttt cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc
     6661 tattctctag ctagaaagta taggaacttc agagcgcttt tgaaaaccaa aagcgctctg
     6721 aagacgcact ttcaaaaaac caaaaacgca ccggactgta acgagctact aaaatattgc
     6781 gaataccgct tccacaaaca ttgctcaaaa gtatctcttt gctatatatc tctgtgctat
     6841 atccctatat aacctaccca tccacctttc gctccttgaa cttgcatcta aactcgacct
     6901 ctacattttt tatgtttatc tctagtatta ctctttagac aaaaaaattg tagtaagaac
     6961 tattcataga gtgaatcgaa aacaatacga aaatgtaaac atttcctata cgtagtatat
     7021 agagacaaaa tagaagaaac cgttcataat tttctgacca atgaagaatc atcaacgcta
     7081 tcactttctg ttcacaaagt atgcgcaatc cacatcggta tagaatataa tcggggatgc
     7141 ctttatcttg aaaaaatgca cccgcagctt cgctagtaat cagtaaacgc gggaagtgga
     7201 gtcaggcttt ttttatggaa gagaaaatag acaccaaagt agccttcttc taaccttaac
     7261 ggacctacag tgcaaaaagt tatcaagaga ctgcattata gagcgcacaa aggagaaaaa
     7321 aagtaatcta agatgctttg ttagaaaaat agcgctctcg ggatgcattt ttgtagaaca
     7381 aaaaagaagt atagattctt tgttggtaaa atagcgctct cgcgttgcat ttctgttctg
     7441 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt
     7501 ttacaaaaat gaagcacaga ttcttcgttg gtaaaatagc gctttcgcgt tgcatttctg
     7561 ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt
     7621 ttgttctaca aaatgaagca cagatgcttc gtt
//
